Topoisomerase I Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11707-50, MB11707-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P11387

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 91 kDa; Observed MW: 91 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody., Immunocytochemistry analysis of Topoisomerase I in Hela using Topoisomerase I antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Topoisomerase I antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: TOP1; DNA topoisomerase 1; DNA topoisomerase I

Immunogen: A synthetic peptide of human Topoisomerase I

Conjugate: Unconjugated

Modification: Unmodified