Phospho-Raf1 (Ser259) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11504-50, MB11504-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P04049

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 73 kDa; Observed MW: 73 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Raf1 in K562, C6, 3T3, Hela lysates using Phospho-Raf1 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1

Conjugate: Unconjugated

Modification: Phosphorylated