Histone H1.3 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11027-50, MB11027-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P16402

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation .

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Histone H1.3 in C6, 3T3, Hela lysates using Histone H1.3 antibody., Immunocytochemistry analysis of Histone H1.3 in Hela using Histone H1.3 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H1.3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: Histone H1c; Histone H1s-2

Immunogen: A synthetic peptide of human Histone H1.3

Conjugate: Unconjugated

Modification: Unmodified